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Sample GSM758321 Query DataSets for GSM758321
Status Public on Jul 18, 2011
Title intestine, BlimpKO, rep7
Sample type RNA
 
Source name Mid-intestine, KO
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Blimp knockout
tissue: mid-intestine
age: postnatal day 7
Treatment protocol Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
Growth protocol Specific pathogen-free (SPF) caging.
Extracted molecule total RNA
Extraction protocol Trizol, followed by Qiagen RNeasy cleanup.
Label Biotin
Label protocol Ambion cRNA synthesis for illumina.
 
Hybridization protocol According to illumina instructions.
Scan protocol According to illumina instructions on a BeadArray 500GX scanner.
Description 5388876090_F.AVG_Signal
VillinCre-Blimpflox
Data processing All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
 
Submission date Jul 11, 2011
Last update date Jul 18, 2011
Contact name Jarom Heijmans
E-mail(s) j.heijmans@amc.nl
Organization name Academic Medical Center
Department Tytgat Institute for liver and intestinal research
Lab van den Brink Group
Street address Meibergdreef 69-71
City Amsterdam
ZIP/Postal code 1105 BK
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE30556 VillinCre-Blimpflox mice postpartum day 7

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 9.5 0.198
ILMN_1212612 9.9 0.18797
ILMN_1212619 -9.8 0.79323
ILMN_1212628 -15 0.93108
ILMN_1212632 14.9 0.11278
ILMN_1212636 916.6 0
ILMN_1212637 799.2 0
ILMN_1212645 -9.5 0.78571
ILMN_1212648 182.8 0
ILMN_1212653 73.1 0.00125
ILMN_1212672 73.5 0.00125
ILMN_1212682 6.4 0.26316
ILMN_1212683 -23.6 0.99373
ILMN_1212685 -13.5 0.88847
ILMN_1212692 2535 0
ILMN_1212693 26.4 0.04637
ILMN_1212695 5.5 0.29073
ILMN_1212698 -11.2 0.83208
ILMN_1212702 17936.4 0
ILMN_1212703 105.6 0

Total number of rows: 25697

Table truncated, full table size 601 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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