|
| Status |
Public on Jul 14, 2011 |
| Title |
[E-MTAB-513] HCT20172, 16 Tissues mixture, 100bp_Stranded_RNA_Seq_FCB_s_4_sequence |
| Sample type |
SRA |
| |
|
| Source name |
16 Tissues mixture, 100bp_Stranded_RNA_Seq_FCB_s_4_sequence
|
| Organism |
Homo sapiens |
| Characteristics |
organismpart: 16 Tissues mixture
material type: molecular_mixture
libraryprep: mRNA-stranded+ DSN
|
| Extracted molecule |
total RNA |
| Extraction protocol |
nucleic_acid_extraction | Final product from mRNA-stranded protocol was normalized by DSN digestion and used for sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
100bp_Stranded_RNA_Seq_FCB_s_4_sequence
1x100 stranded total transcriptome preps; the insert size is variable; could be less than 100 bp. The linker sequence at the end of read will be ATCTCGTATGCCGTCTTCTGCTTG.
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
|
| Data processing |
processed data not provided
|
| |
|
| Submission date |
Jul 12, 2011 |
| Last update date |
May 15, 2019 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
|
| Lab |
ArrayExpress
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE30611 |
[E-MTAB-513] Illumina Human Body Map 2.0 Project |
|
| Relations |
| SRA |
ERX011190 |
