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Sample GSM759532 Query DataSets for GSM759532
Status Public on Jul 14, 2011
Title [E-MTAB-513] HCT20173, 16 Tissues mixture, 100bp_Stranded_RNA_Seq_FCB_s_5_sequence
Sample type SRA
 
Source name 16 Tissues mixture, 100bp_Stranded_RNA_Seq_FCB_s_5_sequence
Organism Homo sapiens
Characteristics organismpart: 16 Tissues mixture
material type: molecular_mixture
libraryprep: totalRNA-stranded+DSN
Extracted molecule total RNA
Extraction protocol nucleic_acid_extraction | 100ng of total RNA was fragmentated, end repared and ligated with illumina small RNA v1.5 adaptors. The ligated RNA was reverse transcribed and PCR amplified with 15 cycles. The PCR product was normalized by DSN digestion and used for sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description 100bp_Stranded_RNA_Seq_FCB_s_5_sequence
1x100 stranded total transcriptome preps; the insert size is variable; could be less than 100 bp. The linker sequence at the end of read will be ATCTCGTATGCCGTCTTCTGCTTG.
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
Data processing processed data not provided
 
Submission date Jul 12, 2011
Last update date May 15, 2019
Organization European Bioinformatics Institute
E-mail(s) miamexpress@ebi.ac.uk
Lab ArrayExpress
Street address Wellcome Trust Genome Campus
City Hinxton
State/province Cambridgeshire
ZIP/Postal code CB10 1SD
Country United Kingdom
 
Platform ID GPL11154
Series (1)
GSE30611 [E-MTAB-513] Illumina Human Body Map 2.0 Project
Relations
SRA ERX011185

Supplementary data files not provided
SRA Run SelectorHelp
Processed data not provided for this record
Raw data are available in SRA

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