|
| Status |
Public on Oct 04, 2012 |
| Title |
Olig2_IP2.Cel |
| Sample type |
RNA |
| |
|
| Source name |
Cortical Oligodendroglia
|
| Organism |
Mus musculus |
| Characteristics |
strain: mix of c57/bl6j, FVB, and Swiss-Webster
cell type: Cortical Oligodendroglia
rna subtype: Ribosome bound RNA
dataset: dataset 1
|
| Treatment protocol |
Either wildtype or induction of tumor (for tumor samples).
|
| Growth protocol |
For first dataset, dissected cortex from adult mice For second dataset, dissected tumors from mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by Qiagen RNeasy Minelute. Translating Ribosome Affinity Purification protocol (see Pubmed: [19013281])
|
| Label |
Biotin
|
| Label protocol |
Affymetric 2 cycle amplification
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocols
|
| Scan protocol |
Standard Affymetrix Protocols
|
| Data processing |
Within replicates GCRMA normalization, using R and Bioconductor. Custom Cdfs: Mouse4302_Mm_ENTREZG, following recommendations as in Pubmed: 20308160
|
| |
|
| Submission date |
Jul 13, 2011 |
| Last update date |
Oct 04, 2012 |
| Contact name |
Joseph D Dougherty |
| Organization name |
Washington University
|
| Department |
Genetics and Psychiatry
|
| Street address |
4566 Scott Ave, box 8232
|
| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL9746 |
| Series (1) |
| GSE30626 |
Candidate pathways for promoting differentiation and quiescence of oligodendrocyte progenitor-like cells in glioblastoma |
|
