|
Status |
Public on Jun 21, 2012 |
Title |
70Z/3 miR-125b #1 |
Sample type |
RNA |
|
|
Source name |
70Z/3, miR-125b
|
Organism |
Mus musculus |
Characteristics |
cell line: 70Z/3 origin: pre-B cell cell type: pre-B lymphoblast disease state: leukemia miRNA transfection: miR-125b
|
Treatment protocol |
Chemically synthesized miRNA duplexes, called pre-miR-125b and miR-Neg, were purchased from Ambion. The cells were transfected with pre-miRNA at a final concentration of 10 nM using Lipofectamin RNAi MAX (Invitrogen) according to the manufacturer's instructions. The medium was replaced 8 hours after transfection. RNA samples were harvested at 48 hours post-transfection.
|
Extracted molecule |
total RNA |
Extraction protocol |
miRNeasy kit (Qiagen). 0.2 ug. RIN number: 9.
|
Label |
Cy3
|
Label protocol |
Agilent Low RNA Input QuickAmp kit. No amplification.
|
|
|
Hybridization protocol |
Agilent : 825.0 ng at 65 degree C during 17 hours.
|
Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
Description |
252800510939-2
|
Data processing |
Feature Extraction data processing.
|
|
|
Submission date |
Jul 13, 2011 |
Last update date |
Jun 21, 2012 |
Contact name |
Kevin Lebrigand |
Organization name |
IPMC/CNRS
|
Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
Street address |
660 route des lucioles
|
City |
Valbonne - Sophia-Antipolis |
ZIP/Postal code |
06560 |
Country |
France |
|
|
Platform ID |
GPL13912 |
Series (1) |
GSE30647 |
Role of miR-125b in B-cell acute lymphoblastic leukemia (B-ALL) |
|