|
| Status |
Public on Jun 21, 2012 |
| Title |
18-81 miR-Neg #2 |
| Sample type |
RNA |
| |
|
| Source name |
18-81, miR-Neg
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 18-81
origin: pre-B cell
cell type: pre-B cell transformed by Abelson-murine leukemia virus (A-MuLV)
miRNA transfection: miR-Neg
|
| Treatment protocol |
Chemically synthesized miRNA duplexes, called pre-miR-125b and miR-Neg, were purchased from Ambion. The cells were transfected with pre-miRNA at a final concentration of 10 nM using Lipofectamin RNAi MAX (Invitrogen) according to the manufacturer's instructions. The medium was replaced 8 hours after transfection. RNA samples were harvested at 48 hours post-transfection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy kit (Qiagen). 0.2 ug. RIN number: 9.
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input QuickAmp kit. No amplification.
|
| |
|
| Hybridization protocol |
Agilent : 825.0 ng at 65 degree C during 17 hours.
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
| Description |
252800510939-7
|
| Data processing |
Feature Extraction data processing.
|
| |
|
| Submission date |
Jul 13, 2011 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE30647 |
Role of miR-125b in B-cell acute lymphoblastic leukemia (B-ALL) |
|
