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Sample GSM759951 Query DataSets for GSM759951
Status Public on Jul 11, 2012
Title Chow Diet, WT mice, rep3
Sample type RNA
 
Source name liver, WT, control diet
Organism Mus musculus
Characteristics background strain: 129SV/C57BL/6
genotype: wild type
tissue: liver
treatment: regular chow diet
treatment duration: 30 days
Treatment protocol Mice were fed a high cholesterol diet (2% cholesterol and 0.5 % sodium cholate ) or a Chow regular diet (Chow) as control.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with the Qiagen RNeasy Kit. Quality control and measurement were performed using a NanoDrop and Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description SAMPLE 3
Replicate 3 of 4.
Data processing Data was processed by using Gene Expression Module v 1.1.1 in Genome Studio V2009.1 from Illumina, Inc. The background noise was subtracted and quantile normalization was applied.
 
Submission date Jul 13, 2011
Last update date Jul 11, 2012
Contact name Daekwan Seo
E-mail(s) daekwan_seo@nih.gov
Phone 301-496-5688
Fax 301-496-0734
Organization name NIH
Department NCI
Lab LEC
Street address 37 Convent Dr. Rm 4140
City Bethesda
State/province MD
ZIP/Postal code 20878
Country USA
 
Platform ID GPL6885
Series (1)
GSE30651 Conditional elimination of c-Met signaling in the liver aggravates oxidative stress and cholestasis, and deregulates lipid metabolism, under a high cholesterol diet

Data table header descriptions
ID_REF
VALUE Quantile-normalized signal intensity without background noise
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1785.31 0
ILMN_2721178 376.7843 0
ILMN_3033922 507.3525 0
ILMN_3092673 3050.615 0
ILMN_2816356 22.13402 0.01002506
ILMN_2808939 1612.393 0
ILMN_2634564 264.2395 0
ILMN_2737647 -5.634346 0.7481203
ILMN_2734484 105.9895 0
ILMN_2952292 34.96852 0.001253133
ILMN_2699078 -2.824654 0.5551379
ILMN_1213681 205.4843 0
ILMN_2735413 478.8155 0
ILMN_2735415 580.4234 0
ILMN_2891688 378.243 0
ILMN_2637698 374.6845 0
ILMN_2674228 116.0431 0
ILMN_2601546 1.7847 0.2869674
ILMN_1230831 -3.297231 0.5877193
ILMN_2848071 1015.857 0

Total number of rows: 25697

Table truncated, full table size 753 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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