|
| Status |
Public on Jul 11, 2012 |
| Title |
High Cholesterol Diet, c-Met KO mice, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
liver, c-Met KO, high cholesterol diet
|
| Organism |
Mus musculus |
| Characteristics |
background strain: 129SV/C57BL/6
genotype: c-Met knockout
tissue: liver
treatment: high cholesterol diet
treatment duration: 30 days
|
| Treatment protocol |
Mice were fed a high cholesterol diet (2% cholesterol and 0.5 % sodium cholate ) or a Chow regular diet (Chow) as control.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiagen RNeasy Kit. Quality control and measurement were performed using a NanoDrop and Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
SAMPLE 14
Replicate 2 of 4.
|
| Data processing |
Data was processed by using Gene Expression Module v 1.1.1 in Genome Studio V2009.1 from Illumina, Inc. The background noise was subtracted and quantile normalization was applied.
|
| |
|
| Submission date |
Jul 13, 2011 |
| Last update date |
Jul 11, 2012 |
| Contact name |
Daekwan Seo |
| E-mail(s) |
daekwan_seo@nih.gov
|
| Phone |
301-496-5688
|
| Fax |
301-496-0734
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
LEC
|
| Street address |
37 Convent Dr. Rm 4140
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20878 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE30651 |
Conditional elimination of c-Met signaling in the liver aggravates oxidative stress and cholestasis, and deregulates lipid metabolism, under a high cholesterol diet |
|
