|
| Status |
Public on Oct 14, 2011 |
| Title |
SMRTmRID E18.5 lungs with PTU, technical replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
E18.5 lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: SMRTmRID in c57Bl6/J bkg
age: E18.5 embryos
tissue: lung
diet: 0.15% PTU
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
technical replicate 2
|
| Data processing |
The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
|
| |
|
| Submission date |
Jul 14, 2011 |
| Last update date |
Oct 14, 2011 |
| Contact name |
Ruth T Yu |
| Organization name |
Salk Institute
|
| Department |
Gene Expression Lab
|
| Lab |
Ronald Evans
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE30661 |
SMRT-RID mutation linked to Type I Pneumocyte Associated Respiratory Distress Syndrome via TR repression |
|
