|
Status |
Public on Jul 19, 2011 |
Title |
control sample 3 |
Sample type |
RNA |
|
|
Source name |
mouse brain
|
Organism |
Mus musculus |
Characteristics |
tissue: brain genotype: APPPS1-FVB
|
Growth protocol |
normal mouse growth
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
GeneChip WT Terminal Labeling and Hybridization Kit” and the “GeneChip Sample Cleanup Module” (Affymetrix)
|
|
|
Hybridization protocol |
Array hybridization was performed according to the supplier’s instructions using the “Ambion WT Expression Kit” (Ambion, Applied Biosystems, Invitrogen) plus the “GeneChip WT Terminal Labeling and Hybridization Kit” and the “GeneChip Sample Cleanup Module” (Affymetrix). For signal enhancement, an antibody amplification was carried out using a biotinylated antistreptavidin antibody (Vector Laboratories.), which was cross-linked by goat IgG (Sigma) followed by a second staining with streptavidin-phycoerythrin conjugate (Molecular Probes, Invitrogen).
|
Scan protocol |
G7 upgraded GeneChip Scanner 3000 (Affymetrix) at 0.7 micron resolution
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Scaled Log2.
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Submission date |
Jul 18, 2011 |
Last update date |
Jul 19, 2011 |
Contact name |
Markus Krohn |
Organization name |
University of Rostock
|
Street address |
Gehlsheimer Str. 20
|
City |
Rostock |
ZIP/Postal code |
18147 |
Country |
Germany |
|
|
Platform ID |
GPL10740 |
Series (1) |
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