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Sample GSM763333 Query DataSets for GSM763333
Status Public on Jul 19, 2011
Title control sample 3
Sample type RNA
 
Source name mouse brain
Organism Mus musculus
Characteristics tissue: brain
genotype: APPPS1-FVB
Growth protocol normal mouse growth
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol GeneChip WT Terminal Labeling and Hybridization Kit” and the “GeneChip Sample Cleanup Module” (Affymetrix)
 
Hybridization protocol Array hybridization was performed according to the supplier’s instructions using the “Ambion WT Expression Kit” (Ambion, Applied Biosystems, Invitrogen) plus the “GeneChip WT Terminal Labeling and Hybridization Kit” and the “GeneChip Sample Cleanup Module” (Affymetrix). For signal enhancement, an antibody amplification was carried out using a biotinylated antistreptavidin antibody (Vector Laboratories.), which was cross-linked by goat IgG (Sigma) followed by a second staining with streptavidin-phycoerythrin conjugate (Molecular Probes, Invitrogen).
Scan protocol G7 upgraded GeneChip Scanner 3000 (Affymetrix) at 0.7 micron resolution
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Scaled Log2.
 
Submission date Jul 18, 2011
Last update date Jul 19, 2011
Contact name Markus Krohn
Organization name University of Rostock
Street address Gehlsheimer Str. 20
City Rostock
ZIP/Postal code 18147
Country Germany
 
Platform ID GPL10740
Series (1)
GSE30762 Mouse expression analysis

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
10338001 10.88
10338002 5.74
10338003 9.41
10338004 8.21
10338005 2.14
10338006 2.36
10338007 2.7
10338008 3.21
10338009 7.71
10338010 2.2
10338011 5.16
10338012 2.28
10338013 1.97
10338014 2.04
10338015 2.02
10338016 6.96
10338017 11.7
10338018 6.21
10338019 4.59
10338020 7.67

Total number of rows: 65535

Table truncated, full table size 893 Kbytes.




Supplementary file Size Download File type/resource
GSM763333.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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