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Sample GSM763550 Query DataSets for GSM763550
Status Public on Sep 01, 2012
Title biological replicate 2: Control (FAC 0µM)
Sample type RNA
 
Source name biological replicate 2: Control (FAC 0µM)
Organism Homo sapiens
Characteristics cell line: MG-63 osteoblastic cell line
Treatment protocol Ferric Ammonium Citrate and/or Desferioxamine were added or not during three days each day when cells reached confluence.
Growth protocol The osteoblast-like MG-63 cells were maintained in minimum essential medium supplemented with 10% fetal bovine serum, 2mM L-Glutamine, 1% non essential amino acids and 1% penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the SV Total RNA Isolation System (Promega®).
Label Cy 3
Label protocol Total RNA (350ng) were amplified and labelled using a One Color Agilent’s low RNA Imput Linear Amplification Kit PLUS according to the manufacturer protocol (Agilent).
 
Hybridization protocol 1,65μg of each sample cRNA were hybridized using in situ Gene Expression Hybridization kit on Agilent Human Whole Genome Microarrays 4 × 44 K at 60°C, during 17h at 10rpm in the dark.
Scan protocol Slides were scanned with the dynamic autofocus G2565BA Agilent scanner.
Probe feature information was extracted using the Agilent Feature Extraction software v.10.7.
Description gene expression after 72h of FAC 0µM treatment (negative control)
314_1.txt
FAC0_2
Data processing Raw intensities were log2-transformed and normalized (median centering within and between arrays) with GeneSpring GX software v11.5.
 
Submission date Jul 19, 2011
Last update date Sep 01, 2012
Contact name DOYARD Mathilde
E-mail(s) mathilde.doyard@univ-rennes1.fr
Phone 33(0)223233860
Fax 33(0)288540137
Organization name INSERM
Department UMRU991
Lab Fer et Foie
Street address rue Henri Le Guilloux
City RENNES
ZIP/Postal code 35000
Country France
 
Platform ID GPL13497
Series (1)
GSE30774 Iron excess limits HHIPL2 gene expression and decreases osteoblastic activity in human MG-63 cells

Data table header descriptions
ID_REF
VALUE normalized log2 signal

Data table
ID_REF VALUE
A_23_P146146 0.028682709
A_23_P42935 7.8439226
A_23_P117082 12.997081
A_23_P2683 8.715322
A_24_P358131 9.040608
A_33_P3367647 0.028682709
A_23_P157316 4.6715493
A_32_P14850 15.564294
A_23_P158596 7.872669
A_23_P350107 10.6147175
A_23_P388190 10.182982
A_23_P106544 11.042273
A_33_P3219745 2.0187979
A_32_P85539 8.946504
A_23_P94998 10.187053
A_33_P3235677 0.028682709
A_23_P417014 4.7187743
A_23_P103905 11.064355
A_24_P497186 10.59419
A_23_P118536 6.5594225

Total number of rows: 34181

Table truncated, full table size 774 Kbytes.




Supplementary file Size Download File type/resource
GSM763550.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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