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Status |
Public on Sep 01, 2012 |
Title |
biological replicate 2: Control (FAC 0µM) |
Sample type |
RNA |
|
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Source name |
biological replicate 2: Control (FAC 0µM)
|
Organism |
Homo sapiens |
Characteristics |
cell line: MG-63 osteoblastic cell line
|
Treatment protocol |
Ferric Ammonium Citrate and/or Desferioxamine were added or not during three days each day when cells reached confluence.
|
Growth protocol |
The osteoblast-like MG-63 cells were maintained in minimum essential medium supplemented with 10% fetal bovine serum, 2mM L-Glutamine, 1% non essential amino acids and 1% penicillin/streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using the SV Total RNA Isolation System (Promega®).
|
Label |
Cy 3
|
Label protocol |
Total RNA (350ng) were amplified and labelled using a One Color Agilent’s low RNA Imput Linear Amplification Kit PLUS according to the manufacturer protocol (Agilent).
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Hybridization protocol |
1,65μg of each sample cRNA were hybridized using in situ Gene Expression Hybridization kit on Agilent Human Whole Genome Microarrays 4 × 44 K at 60°C, during 17h at 10rpm in the dark.
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Scan protocol |
Slides were scanned with the dynamic autofocus G2565BA Agilent scanner. Probe feature information was extracted using the Agilent Feature Extraction software v.10.7.
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Description |
gene expression after 72h of FAC 0µM treatment (negative control) 314_1.txt FAC0_2
|
Data processing |
Raw intensities were log2-transformed and normalized (median centering within and between arrays) with GeneSpring GX software v11.5.
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Submission date |
Jul 19, 2011 |
Last update date |
Sep 01, 2012 |
Contact name |
DOYARD Mathilde |
E-mail(s) |
mathilde.doyard@univ-rennes1.fr
|
Phone |
33(0)223233860
|
Fax |
33(0)288540137
|
Organization name |
INSERM
|
Department |
UMRU991
|
Lab |
Fer et Foie
|
Street address |
rue Henri Le Guilloux
|
City |
RENNES |
ZIP/Postal code |
35000 |
Country |
France |
|
|
Platform ID |
GPL13497 |
Series (1) |
GSE30774 |
Iron excess limits HHIPL2 gene expression and decreases osteoblastic activity in human MG-63 cells |
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