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Sample GSM763556 Query DataSets for GSM763556
Status Public on Sep 01, 2012
Title biological replicate 3: 72h FAC 5µM
Sample type RNA
 
Source name biological replicate 3: 72h FAC 5µM
Organism Homo sapiens
Characteristics cell line: MG-63 osteoblastic cell line
Treatment protocol Ferric Ammonium Citrate and/or Desferioxamine were added or not during three days each day when cells reached confluence.
Growth protocol The osteoblast-like MG-63 cells were maintained in minimum essential medium supplemented with 10% fetal bovine serum, 2mM L-Glutamine, 1% non essential amino acids and 1% penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the SV Total RNA Isolation System (Promega®).
Label Cy 3
Label protocol Total RNA (350ng) were amplified and labelled using a One Color Agilent’s low RNA Imput Linear Amplification Kit PLUS according to the manufacturer protocol (Agilent).
 
Hybridization protocol 1,65μg of each sample cRNA were hybridized using in situ Gene Expression Hybridization kit on Agilent Human Whole Genome Microarrays 4 × 44 K at 60°C, during 17h at 10rpm in the dark.
Scan protocol Slides were scanned with the dynamic autofocus G2565BA Agilent scanner.
Probe feature information was extracted using the Agilent Feature Extraction software v.10.7.
Description gene expression after 72h of FAC 5µM treatment
264_1.txt
FAC5_3
Data processing Raw intensities were log2-transformed and normalized (median centering within and between arrays) with GeneSpring GX software v11.5.
 
Submission date Jul 19, 2011
Last update date Sep 01, 2012
Contact name DOYARD Mathilde
E-mail(s) mathilde.doyard@univ-rennes1.fr
Phone 33(0)223233860
Fax 33(0)288540137
Organization name INSERM
Department UMRU991
Lab Fer et Foie
Street address rue Henri Le Guilloux
City RENNES
ZIP/Postal code 35000
Country France
 
Platform ID GPL13497
Series (1)
GSE30774 Iron excess limits HHIPL2 gene expression and decreases osteoblastic activity in human MG-63 cells

Data table header descriptions
ID_REF
VALUE normalized log2 signal

Data table
ID_REF VALUE
A_23_P146146 1.4026233
A_23_P42935 7.8063173
A_23_P117082 13.072391
A_23_P2683 8.338487
A_24_P358131 8.318254
A_33_P3367647 -0.08538246
A_23_P157316 4.1257935
A_32_P14850 15.457054
A_23_P158596 8.34767
A_23_P350107 10.900744
A_23_P388190 9.556451
A_23_P106544 11.002513
A_33_P3219745 -0.08538246
A_32_P85539 9.181738
A_23_P94998 9.295415
A_33_P3235677 -0.08538246
A_23_P417014 5.057107
A_23_P103905 11.126967
A_24_P497186 10.754783
A_23_P118536 7.0474534

Total number of rows: 34181

Table truncated, full table size 774 Kbytes.




Supplementary file Size Download File type/resource
GSM763556.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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