|
| Status |
Public on Sep 01, 2012 |
| Title |
biological replicate 1: 72h DFO 20µM |
| Sample type |
RNA |
| |
|
| Source name |
biological replicate 1: 72h DFO 20µM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MG-63 osteoblastic cell line
|
| Treatment protocol |
Ferric Ammonium Citrate and/or Desferioxamine were added or not during three days each day when cells reached confluence.
|
| Growth protocol |
The osteoblast-like MG-63 cells were maintained in minimum essential medium supplemented with 10% fetal bovine serum, 2mM L-Glutamine, 1% non essential amino acids and 1% penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the SV Total RNA Isolation System (Promega®).
|
| Label |
Cy 3
|
| Label protocol |
Total RNA (350ng) were amplified and labelled using a One Color Agilent’s low RNA Imput Linear Amplification Kit PLUS according to the manufacturer protocol (Agilent).
|
| |
|
| Hybridization protocol |
1,65μg of each sample cRNA were hybridized using in situ Gene Expression Hybridization kit on Agilent Human Whole Genome Microarrays 4 × 44 K at 60°C, during 17h at 10rpm in the dark.
|
| Scan protocol |
Slides were scanned with the dynamic autofocus G2565BA Agilent scanner.
Probe feature information was extracted using the Agilent Feature Extraction software v.10.7.
|
| Description |
gene expression after 72h of DFO 20µM treatment
262_3.txt
DFO_1
|
| Data processing |
Raw intensities were log2-transformed and normalized (median centering within and between arrays) with GeneSpring GX software v11.5.
|
| |
|
| Submission date |
Jul 19, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
DOYARD Mathilde |
| E-mail(s) |
mathilde.doyard@univ-rennes1.fr
|
| Phone |
33(0)223233860
|
| Fax |
33(0)288540137
|
| Organization name |
INSERM
|
| Department |
UMRU991
|
| Lab |
Fer et Foie
|
| Street address |
rue Henri Le Guilloux
|
| City |
RENNES |
| ZIP/Postal code |
35000 |
| Country |
France |
| |
|
| Platform ID |
GPL13497 |
| Series (1) |
| GSE30774 |
Iron excess limits HHIPL2 gene expression and decreases osteoblastic activity in human MG-63 cells |
|
