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Sample GSM763569 Query DataSets for GSM763569
Status Public on Sep 01, 2012
Title biological replicate 1: 72h DFO 20µM+FAC 20µM
Sample type RNA
 
Source name biological replicate 1: 72h DFO 20µM+FAC 20µM
Organism Homo sapiens
Characteristics cell line: MG-63 osteoblastic cell line
Treatment protocol Ferric Ammonium Citrate and/or Desferioxamine were added or not during three days each day when cells reached confluence.
Growth protocol The osteoblast-like MG-63 cells were maintained in minimum essential medium supplemented with 10% fetal bovine serum, 2mM L-Glutamine, 1% non essential amino acids and 1% penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the SV Total RNA Isolation System (Promega®).
Label Cy 3
Label protocol Total RNA (350ng) were amplified and labelled using a One Color Agilent’s low RNA Imput Linear Amplification Kit PLUS according to the manufacturer protocol (Agilent).
 
Hybridization protocol 1,65μg of each sample cRNA were hybridized using in situ Gene Expression Hybridization kit on Agilent Human Whole Genome Microarrays 4 × 44 K at 60°C, during 17h at 10rpm in the dark.
Scan protocol Slides were scanned with the dynamic autofocus G2565BA Agilent scanner.
Probe feature information was extracted using the Agilent Feature Extraction software v.10.7.
Description gene expression after 72h of DFO 20µM+FAC 20µM treatment
127_4.txt
DFO+FAC_1
Data processing Raw intensities were log2-transformed and normalized (median centering within and between arrays) with GeneSpring GX software v11.5.
 
Submission date Jul 19, 2011
Last update date Sep 01, 2012
Contact name DOYARD Mathilde
E-mail(s) mathilde.doyard@univ-rennes1.fr
Phone 33(0)223233860
Fax 33(0)288540137
Organization name INSERM
Department UMRU991
Lab Fer et Foie
Street address rue Henri Le Guilloux
City RENNES
ZIP/Postal code 35000
Country France
 
Platform ID GPL13497
Series (1)
GSE30774 Iron excess limits HHIPL2 gene expression and decreases osteoblastic activity in human MG-63 cells

Data table header descriptions
ID_REF
VALUE normalized log2 signal

Data table
ID_REF VALUE
A_23_P146146 0.09919834
A_23_P42935 7.922316
A_23_P117082 13.433886
A_23_P2683 8.928339
A_24_P358131 8.846528
A_33_P3367647 1.7448663
A_23_P157316 4.8048677
A_32_P14850 15.71812
A_23_P158596 8.079138
A_23_P350107 10.7436075
A_23_P388190 9.898688
A_23_P106544 11.456541
A_33_P3219745 0.657219
A_32_P85539 9.353109
A_23_P94998 10.091712
A_33_P3235677 0.88465524
A_23_P417014 4.8446646
A_23_P103905 11.221737
A_24_P497186 10.641746
A_23_P118536 6.7529693

Total number of rows: 34181

Table truncated, full table size 769 Kbytes.




Supplementary file Size Download File type/resource
GSM763569.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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