|
| Status |
Public on Jul 26, 2011 |
| Title |
Elongin A-/- (EloA-/-), +RA |
| Sample type |
RNA |
| |
|
| Source name |
Elongin A-/- EBs, 2 μM RA
|
| Organism |
Mus musculus |
| Characteristics |
sample type: embryoid bodies derived from Elongin A-/- ES cells
|
| Treatment protocol |
EBs were treated 2 μM retinoic acid for 4 days.
|
| Growth protocol |
DMEM/F12 (1:1) containing 15% FBS and N2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from EBs using a RNeasy mini kit (Qiagen) according to the manufacturer’s instructions, and the quality was determined using an Agilent Bioanalyzer 2100 (Agilent Technologies).
|
| Label |
DIG-UTP
|
| Label protocol |
RNA (1 μg) was transcribed to DIG-labeled cRNA using an Applied Biosystems Chemiluminescent NanoAmpTM RT-IVT Labeling Kit (Applied Biosystems) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Microarray hybridization and processing were performed according to Applied Biosystems protocols.
|
| Scan protocol |
Chemiluminescence detection, imaging, auto-gridding, and image analysis were performed according to Applied Biosystems protocols using the 1700 Chemiluminescent Microarray Analyzer software (Version 1.0.3).
|
| Description |
Gene expression of RA-treated EBs derived from Elongin A-/- ES cells
|
| Data processing |
Signal intensities across microarrays were quantile normalized.
|
| |
|
| Submission date |
Jul 25, 2011 |
| Last update date |
Jul 26, 2011 |
| Contact name |
Takashi yasukawa |
| E-mail(s) |
tyasu@kochi-u.ac.jp
|
| Organization name |
Kochi Medical School
|
| Department |
Functional Genomics
|
| Street address |
Kohasu, Oko-cho
|
| City |
Nankoku |
| State/province |
Kochi |
| ZIP/Postal code |
783-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE30911 |
Gene expression profiling of retinoic acid (RA)-treated embryoid bodies (EBs) derived from Elongin A+/+ and Elongin A-/- ES cells. |
|
