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Status |
Public on Jul 26, 2011 |
Title |
Elongin A-/- (EloA-/-), +RA |
Sample type |
RNA |
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Source name |
Elongin A-/- EBs, 2 μM RA
|
Organism |
Mus musculus |
Characteristics |
sample type: embryoid bodies derived from Elongin A-/- ES cells
|
Treatment protocol |
EBs were treated 2 μM retinoic acid for 4 days.
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Growth protocol |
DMEM/F12 (1:1) containing 15% FBS and N2
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from EBs using a RNeasy mini kit (Qiagen) according to the manufacturer’s instructions, and the quality was determined using an Agilent Bioanalyzer 2100 (Agilent Technologies).
|
Label |
DIG-UTP
|
Label protocol |
RNA (1 μg) was transcribed to DIG-labeled cRNA using an Applied Biosystems Chemiluminescent NanoAmpTM RT-IVT Labeling Kit (Applied Biosystems) according to the manufacturer’s instructions.
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Hybridization protocol |
Microarray hybridization and processing were performed according to Applied Biosystems protocols.
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Scan protocol |
Chemiluminescence detection, imaging, auto-gridding, and image analysis were performed according to Applied Biosystems protocols using the 1700 Chemiluminescent Microarray Analyzer software (Version 1.0.3).
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Description |
Gene expression of RA-treated EBs derived from Elongin A-/- ES cells
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Data processing |
Signal intensities across microarrays were quantile normalized.
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Submission date |
Jul 25, 2011 |
Last update date |
Jul 26, 2011 |
Contact name |
Takashi yasukawa |
E-mail(s) |
tyasu@kochi-u.ac.jp
|
Organization name |
Kochi Medical School
|
Department |
Functional Genomics
|
Street address |
Kohasu, Oko-cho
|
City |
Nankoku |
State/province |
Kochi |
ZIP/Postal code |
783-8505 |
Country |
Japan |
|
|
Platform ID |
GPL2995 |
Series (1) |
GSE30911 |
Gene expression profiling of retinoic acid (RA)-treated embryoid bodies (EBs) derived from Elongin A+/+ and Elongin A-/- ES cells. |
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