|
| Status |
Public on Jul 30, 2011 |
| Title |
Partenogenetic mouse embryonic stem cells_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
parthenogenetic mouse embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse oocytes
strain: B6D2F1 hybrid mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R. The filtering parameter, 50% sample of total are Detection pval < 0.05, used to derive our reduced data set.
|
| |
|
| Submission date |
Jul 29, 2011 |
| Last update date |
Jul 30, 2011 |
| Contact name |
Jaeku Kang |
| E-mail(s) |
jaeku@konyang.ac.kr
|
| Phone |
82-42-600-6415
|
| Organization name |
College of Medicine, Konyang University
|
| Department |
Department of Pharmacology
|
| Street address |
685, Gasuwon-dong
|
| City |
Seu-gu |
| State/province |
Daejeon |
| ZIP/Postal code |
302-718 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31031 |
The expression profiles of genes using genetically modified parthenogenetic mouse embryonic stem cells |
|
