|
| Status |
Public on Mar 22, 2012 |
| Title |
3WD, Liposarcoma (Slide 29) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Liposarcoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liposarcoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Hy3
|
| Label protocol |
The samples are labeled using the miRCURY Hy3/Hy5Power labeling kit
|
| |
|
| Channel 2 |
| Source name |
common reference
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: common reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Hy5
|
| Label protocol |
The samples are labeled using the miRCURY Hy3/Hy5Power labeling kit
|
| |
|
| |
| Hybridization protocol |
The samples are hybridized on the miRCURY LNA Array (v.11.0)
|
| Scan protocol |
Scanned on an Axon GenePix 4000B. Images were quantified using GenePix pro V6.0.
|
| Description |
3WD, Liposarcoma (Slide 29)
|
| Data processing |
Within-slide normalisation of Hy3/Hy5 ratios (log2 transformed) by background subtraction and with a global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
| |
|
| Submission date |
Jul 29, 2011 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE31045 |
MicroRNAs differentially expressed in liposarcoma |
|
