|
Status |
Public on Mar 22, 2012 |
Title |
8DD, Liposarcoma (Slide 35) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Liposarcoma
|
Organism |
Homo sapiens |
Characteristics |
tissue: Liposarcoma
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
Label |
Hy3
|
Label protocol |
The samples are labeled using the miRCURY Hy3/Hy5Power labeling kit
|
|
|
Channel 2 |
Source name |
common reference
|
Organism |
Homo sapiens |
Characteristics |
sample type: common reference
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
Label |
Hy5
|
Label protocol |
The samples are labeled using the miRCURY Hy3/Hy5Power labeling kit
|
|
|
|
Hybridization protocol |
The samples are hybridized on the miRCURY LNA Array (v.11.0)
|
Scan protocol |
Scanned on an Axon GenePix 4000B. Images were quantified using GenePix pro V6.0.
|
Description |
8DD, Liposarcoma (Slide 35)
|
Data processing |
Within-slide normalisation of Hy3/Hy5 ratios (log2 transformed) by background subtraction and with a global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
|
|
Submission date |
Jul 29, 2011 |
Last update date |
Mar 22, 2012 |
Contact name |
Chad Creighton |
E-mail(s) |
creighto@bcm.tmc.edu
|
Organization name |
Baylor College of Medicine
|
Department |
Biostatistics, Ducan Cancer Center
|
Street address |
One Baylor Plaza, Mail Stop: BCM305
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL7723 |
Series (1) |
GSE31045 |
MicroRNAs differentially expressed in liposarcoma |
|