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Sample GSM769883 Query DataSets for GSM769883
Status Public on Nov 05, 2011
Title Control_siRNA
Sample type RNA
 
Source name HepG2 cells
Organism Homo sapiens
Characteristics cell type: HepG2
sirna: Control
Extracted molecule total RNA
Extraction protocol HepG2 cells were transfected with control siRNA or siRNA against MCPIP1. After transfection, total RNA was prepared using Trizol (Invitrogen).
Label Hy3
Label protocol A total of 1 ug of total RNA was used for each sample. MiRCURY™ LNA microRNA Power labeling Kit (Exiqon, Vedbaek, Denmark) was used following the manufacturers’ recommendations. Spike-ins (used as control probes) were added in equal amounts to each reaction and labeled.
 
Hybridization protocol Hybridization buffer was added to each labelled samples and denatured at 95C for 2min and then cooled. The samples were then hybridized to miRCURY LNA v.11 microarray at 56C for 16h in agilent hybridization oven rotated at 20 RPM. The following day, the slides were washed, dried, and scanned immediately.
Scan protocol The miRNA arrays were scanned using a GenePix 4000B scanner (Molecular Devices), and data were analyzed using Array-Pro Analyzer Version 4.5 (Media Cybernetics).
Data processing The signals across samples were normalized using a factor derived from the mean of the signal intensity of several house-keeping small RNAs.
 
Submission date Aug 01, 2011
Last update date Nov 05, 2011
Contact name Hiroshi I. Suzuki
E-mail(s) hisuzuki-tky@umin.ac.jp
Organization name Nagoya University
Department DIvision of Molecular Oncology, Graduate School of Medicine
Street address 65 Tsurumai-cho, Showa-ku
City Nagoya
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL7723
Series (1)
GSE31091 Regulation of miRNA biogenesis by MCPIP1

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
4040 76.21496825
4610 67.32722925
4700 82.607484
5250 141.2969745
5730 117.7213375
5740 3807.800159
6880 78.05812125
9938 75.0015925
10138 68.17356675
10306 117.3742038
10482 60.6767515
10916 46.9800955
10919 283.7945863
10923 168.4323253
10925 243.812102
10928 204.7834395
10936 88.75796175
10937 47.81369425
10942 198.825637
10943 95.4530255

Total number of rows: 840

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM769883_4_Z1.txt.gz 240.4 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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