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Sample GSM769884 Query DataSets for GSM769884
Status Public on Nov 05, 2011
Title MCPIP1_siRNA
Sample type RNA
 
Source name HepG2 cells
Organism Homo sapiens
Characteristics cell type: HepG2
sirna: MCPIP1
Extracted molecule total RNA
Extraction protocol HepG2 cells were transfected with control siRNA or siRNA against MCPIP1. After transfection, total RNA was prepared using Trizol (Invitrogen).
Label Hy3
Label protocol A total of 1 ug of total RNA was used for each sample. MiRCURY™ LNA microRNA Power labeling Kit (Exiqon, Vedbaek, Denmark) was used following the manufacturers’ recommendations. Spike-ins (used as control probes) were added in equal amounts to each reaction and labeled.
 
Hybridization protocol Hybridization buffer was added to each labelled samples and denatured at 95C for 2min and then cooled. The samples were then hybridized to miRCURY LNA v.11 microarray at 56C for 16h in agilent hybridization oven rotated at 20 RPM. The following day, the slides were washed, dried, and scanned immediately.
Scan protocol The miRNA arrays were scanned using a GenePix 4000B scanner (Molecular Devices), and data were analyzed using Array-Pro Analyzer Version 4.5 (Media Cybernetics).
Data processing The signals across samples were normalized using a factor derived from the mean of the signal intensity of several house-keeping small RNAs.
 
Submission date Aug 01, 2011
Last update date Nov 05, 2011
Contact name Hiroshi I. Suzuki
E-mail(s) hisuzuki-tky@umin.ac.jp
Organization name Nagoya University
Department DIvision of Molecular Oncology, Graduate School of Medicine
Street address 65 Tsurumai-cho, Showa-ku
City Nagoya
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL7723
Series (1)
GSE31091 Regulation of miRNA biogenesis by MCPIP1

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
4040 85.541401
4610 65.44745225
4700 90.3638535
5250 180.901274
5730 147.0907643
5740 5741.401274
6880 83.705414
9938 76.450637
10138 68.22611475
10306 139.6759553
10482 63.46496825
10916 48.4713375
10919 413.4243633
10923 243.0493633
10925 375.1019108
10928 223.1552548
10936 104.3320065
10937 61.39171975
10942 230.0326433
10943 100.7914013

Total number of rows: 840

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM769884_2_Z2.txt.gz 241.0 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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