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Sample GSM771196 Query DataSets for GSM771196
Status Public on Aug 03, 2011
Title HN05.192T
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics patient identifier: 10
disease state: squamous cell carcinoma of the tongue
tissue location: tongue
Stage: IV
grade: keratinizing mod diff
site: tumor
site detail: tumor
recurrence: yes
time.to.recurrence.or.last.follow-up.months: 1.83
site.of.recurrence: regional and distant
outcome: DOD
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from all tissues using Trizol reagent (Life Technologies, Inc., Burlington, ON, Canada), followed by purification using the Qiagen RNeasy kit/DNase RNase-free set (Qiagen, Valencia, CA, USA), according to manufacturer’s instructions. RNA was quantified by spectrophotometry and its quality was assessed using the 2100 Bioanalyzer (Firmware v.A.01.16, Agilent Technologies, Canada). All samples were of sufficient quantity and quality for arrays and quantitative real-time PCR (QRT-PCR) analyses.
Label biotin
Label protocol Amplification and biotin labeling of antisense cRNA was performed using the Enzo BioArray High YieldTM RNA transcript labeling kit (Enzo Diagnostics, Farmingdale, NY, USA), according to the manufacturer’s instructions.
 
Hybridization protocol Labeling and hybridization to arrays were performed by The Centre for Applied Genomics, Medical and Related Sciences Centre (MaRS), Toronto, ON, Canada, using standard Affymetrix procedures.
Scan protocol Microarray slides were scanned using the GeneArray 2500 scanner (Agilent Technologies).
Description U133Plus2_073008L_SR15_HNO5 192T

# outcome: AWD (alive with disease); ANED (alive, no evidence of disease); DOD (dead of disease); DOC (dead of other causes).
Data processing Data were processed using the BrainArray Entrez-gene centric alternative CDF, with GC-RMA background correction and normalization.
 
Submission date Aug 02, 2011
Last update date Aug 03, 2011
Contact name Levi Waldron
E-mail(s) lwaldron.research@gmail.com
Organization name Harvard School of Publich Health
Department Biostatistics
Street address 655 Huntington Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL10526
Series (1)
GSE31056 A gene signature in histologically normal surgical margins is predictive of oral carcinoma recurrence

Data table header descriptions
ID_REF
VALUE Log2 GC-RMA signal using Entrez-gene centered alternative chip definition file

Data table
ID_REF VALUE
10000_at 2.29242561
10001_at 9.719293149
10002_at 2.115190777
10003_at 2.445251765
100048912_at 2.115190777
100049716_at 3.837677295
10004_at 3.057196417
10005_at 8.601506907
10006_at 10.67060829
10007_at 10.14711996
10008_at 2.584457838
100093630_at 9.321372056
10009_at 8.242408352
1000_at 5.644126134
100101467_at 2.941822901
10010_at 9.835989437
100113377_at 2.232077049
100113407_at 2.365946993
10011_at 9.758338331
100124700_at 2.117775989

Total number of rows: 17788

Table truncated, full table size 361 Kbytes.




Supplementary file Size Download File type/resource
GSM771196.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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