|
| Status |
Public on Aug 18, 2011 |
| Title |
Mouse ES-derived motor neuron_GFP med_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic stem cell-derived motor neurons
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Hb9::GFP-transgenic
gfp fluorescence: GFP-medium
cell line: V6.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared by double amplification with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
FACS-purified and double-amplified
|
| Data processing |
The data were normalized using cubic spline normalization in BeadStudio.
|
| |
|
| Submission date |
Aug 02, 2011 |
| Last update date |
Aug 18, 2011 |
| Contact name |
Esther Yesde Son |
| E-mail(s) |
son@fas.harvard.edu
|
| Phone |
617-496-5611
|
| Fax |
617-384-5611
|
| Organization name |
Harvard University
|
| Department |
Stem Cells and Regenerative Biology
|
| Lab |
Eggan
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31160 |
Global gene expression analysis of three different types of mouse motor neurons |
|
