|
| Status |
Public on Dec 28, 2011 |
| Title |
ChIP-Seq analysis of H3K27me3 in human CD45RO CD8 cells; DNA_Lib 544 |
| Sample type |
SRA |
| |
|
| Source name |
Memory CD8+ T cells; DNA_Lib 544
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: BioSam 182
sample common name: CD8 Memory Primary Cells
donor_health_status: presumed normal
molecule: genomic DNA
disease: presumed normal
markers: CD8+
passage_if_expanded: NA
cell_type: CD45RO CD8
donor_ethnicity: NA
donor_sex: Unknown
biomaterial_type: Primary Cell
donor_id: Donor 100 7 pooled leukopaks Jan 7 2011
biomaterial_provider: Dana Farber
donor_age: NA
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_antibody: H3K27me3
chip_protocol_chromatin_amount: 0.5 to 3 million cells
extraction_protocol: cell/nuclei lysis
chip_protocol_antibody_amount: ~1 ug
chip_protocol_bead_amount: 10 ul bed volume
chip_protocol: Bernstein_BROAD_ENCODE_protocol
extraction_protocol_sonication_cycles: 1
chip_antibody_catalog: 07-449
chip_antibody_provider: Millipore
chip_antibody_lot: DAM1514011
experiment_type: Histone H3K27me3
extraction_protocol_type_of_sonicator: Branson
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
sample_term_id: CL_0000909
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: DNA_Lib 544
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.8568
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.9468
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM772878_BI.CD8_Memory_Primary_Cells.H3K27me3.Donor_100_7_pooled_leukopaks_Jan_7_2011.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 544-DNA_Lib 544-DNA_Lib 544.hg19.level.1.release.5
ANALYSIS TITLE: Mapping of CD8 Memory Primary Cells Histone H3K27me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by Histone H3K27me3 ChIP-Seq on CD8 Memory Primary Cells, Donor Donor 100 7 pooled leukopaks Jan 7 2011, were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.10636
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 5
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 26,412,322
NUMBER_OF_H3K27me3_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 49
FINDPEAKS_SCORE: 0.0071
FINDPEAKS_PERCENTILE: 10
HOTSPOT_SCORE: 0.0768
HOTSPOT_PERCENTILE: 10
IROC_SCORE: 0.6733
IROC_PERCENTILE: 24
POISSON_SCORE: 0.1995
POISSON_PERCENTILE: 27
MAXIMUM_REPLICATE_CORRELATION: 0.82
**********************************************************************
ANALYSIS FILE NAME: GSM772878_BI.CD8_Memory_Primary_Cells.H3K27me3.Donor_100_7_pooled_leukopaks_Jan_7_2011.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 544-DNA_Lib 544-DNA_Lib 544.hg19.level.2.release.5
ANALYSIS TITLE: Raw Signal Density Graphs of CD8 Memory Primary Cells Histone H3K27me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina Histone H3K27me3 ChIP-Seq read mappings from CD8 Memory Primary Cells, Donor Donor 100 7 pooled leukopaks Jan 7 2011, were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.10900
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 5
BROWSER_TRACK_NAME: CD8M H3K27me3 00 44
BROWSER_TRACK_DESCRIPTION: BI CD8 Memory Primary Cells Histone H3K27me3 Donor 100 7 pooled leukopaks Jan 7 2011 Library DNA_Lib 544 EA Release 5
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 26,412,322
NUMBER_OF_H3K27me3_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 49
FINDPEAKS_SCORE: 0.0071
FINDPEAKS_PERCENTILE: 10
HOTSPOT_SCORE: 0.0768
HOTSPOT_PERCENTILE: 10
IROC_SCORE: 0.6733
IROC_PERCENTILE: 24
POISSON_SCORE: 0.1995
POISSON_PERCENTILE: 27
MAXIMUM_REPLICATE_CORRELATION: 0.82
**********************************************************************
|
| |
|
| Submission date |
Aug 03, 2011 |
| Last update date |
Jan 29, 2015 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
| Relations |
| BioSample |
SAMN00691481 |
| Named Annotation |
GSM772878_BI.CD8_Memory_Primary_Cells.H3K27me3.Donor_100_7_pooled_leukopaks_Jan_7_2011.wig.gz |
