|
Status |
Public on Jul 28, 2014 |
Title |
frontal cortices_male control_6 month_rep3 |
Sample type |
RNA |
|
|
Source name |
male control_frontal cortices
|
Organism |
Mus musculus |
Characteristics |
strain: BALB/c gender: Male tissue: Frontal Cortex infection: none (control)
|
Treatment protocol |
Mice of each gender were either mock-infected with sterile DPBS or infected with 400 tachyzoites (2 parasites/μl) intraperitoneally.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was harvested using the RNeasy reagent . The RNA concentration and integrity was determined using Agilent BioAnalyzer.
|
Label |
biotin
|
Label protocol |
Total RNA was used to generate biotin labeled cRNA using the Illumina TotalPrep RNA Amplification Kit.
|
|
|
Hybridization protocol |
Biotinylated cRNAs were purified, fragmented, and subsequently hybridized to Illumina Mouse Ref-8 v2.0 Expression BeadChips. Each BeadChip has 24,000 well-annotated RefSeq transcripts with approximately 30-fold redundancy.
|
Scan protocol |
The arrays were scanned using an Illumina BeadStation 500X Genetic Analysis Systems scanner and the image data extracted using Illumina GenomeStudio software, version 3.0.
|
Description |
Mm CTX Male Control #3
|
Data processing |
Data were analyzed using GeneSpring software v. 11.0.2. Raw data were imported into GeneSpring and normalized using global normalization.
|
|
|
Submission date |
Aug 04, 2011 |
Last update date |
Jul 28, 2014 |
Contact name |
JC xiao |
E-mail(s) |
jxiao4@jhmi.edu
|
Organization name |
Johns Hopkins University
|
Street address |
600 N. Wolfe Street, Blalock 1109
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21287 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE31207 |
The Sex-Specific Changes in Gene Expression and Behavior Induced by Chronic Toxoplasma Infection in Mice |
|