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Sample GSM773511 Query DataSets for GSM773511
Status Public on Jul 28, 2014
Title frontal cortices_male control_6 month_rep3
Sample type RNA
 
Source name male control_frontal cortices
Organism Mus musculus
Characteristics strain: BALB/c
gender: Male
tissue: Frontal Cortex
infection: none (control)
Treatment protocol Mice of each gender were either mock-infected with sterile DPBS or infected with 400 tachyzoites (2 parasites/μl) intraperitoneally.
Extracted molecule total RNA
Extraction protocol Total RNA was harvested using the RNeasy reagent . The RNA concentration and integrity was determined using Agilent BioAnalyzer.
Label biotin
Label protocol Total RNA was used to generate biotin labeled cRNA using the Illumina TotalPrep RNA Amplification Kit.
 
Hybridization protocol Biotinylated cRNAs were purified, fragmented, and subsequently hybridized to Illumina Mouse Ref-8 v2.0 Expression BeadChips. Each BeadChip has 24,000 well-annotated RefSeq transcripts with approximately 30-fold redundancy.
Scan protocol The arrays were scanned using an Illumina BeadStation 500X Genetic Analysis Systems scanner and the image data extracted using Illumina GenomeStudio software, version 3.0.
Description Mm CTX Male Control #3
Data processing Data were analyzed using GeneSpring software v. 11.0.2. Raw data were imported into GeneSpring and normalized using global normalization.
 
Submission date Aug 04, 2011
Last update date Jul 28, 2014
Contact name JC xiao
E-mail(s) jxiao4@jhmi.edu
Organization name Johns Hopkins University
Street address 600 N. Wolfe Street, Blalock 1109
City Baltimore
State/province MD
ZIP/Postal code 21287
Country USA
 
Platform ID GPL6885
Series (1)
GSE31207 The Sex-Specific Changes in Gene Expression and Behavior Induced by Chronic Toxoplasma Infection in Mice

Data table header descriptions
ID_REF
VALUE The normalized data were used to identify changes in gene expression in the 2 group comparisons: male infected vs male control and female infected vs female control.

Data table
ID_REF VALUE
ILMN_2607609 -0.3799553
ILMN_1238674 0
ILMN_3062534 0.47693872
ILMN_3140158 -0.16305685
ILMN_2674533 0.02417183
ILMN_2680307 -0.41773129
ILMN_2935307 0
ILMN_2910106 -0.19641447
ILMN_1215893 -0.59052134
ILMN_2732092 -0.36693048
ILMN_2603725 -0.18016815
ILMN_2664884 -0.70172167
ILMN_3003575 0
ILMN_1229397 0.11220312
ILMN_1225873 -0.20360947
ILMN_2899591 -0.19295597
ILMN_2658355 0
ILMN_2682763 -0.7908976
ILMN_2521511 0
ILMN_2691286 1.4329594

Total number of rows: 25697

Table truncated, full table size 553 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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