NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM773514 Query DataSets for GSM773514
Status Public on Jul 28, 2014
Title frontal cortices_male infection_6 month_rep2
Sample type RNA
 
Source name male infection_frontal cortices
Organism Mus musculus
Characteristics strain: BALB/c
gender: Male
tissue: Frontal Cortex
infection: 400 tachyzoites of Toxoplasma pru strain
Treatment protocol Mice of each gender were either mock-infected with sterile DPBS or infected with 400 tachyzoites (2 parasites/μl) intraperitoneally.
Extracted molecule total RNA
Extraction protocol Total RNA was harvested using the RNeasy reagent . The RNA concentration and integrity was determined using Agilent BioAnalyzer.
Label biotin
Label protocol Total RNA was used to generate biotin labeled cRNA using the Illumina TotalPrep RNA Amplification Kit.
 
Hybridization protocol Biotinylated cRNAs were purified, fragmented, and subsequently hybridized to Illumina Mouse Ref-8 v2.0 Expression BeadChips. Each BeadChip has 24,000 well-annotated RefSeq transcripts with approximately 30-fold redundancy.
Scan protocol The arrays were scanned using an Illumina BeadStation 500X Genetic Analysis Systems scanner and the image data extracted using Illumina GenomeStudio software, version 3.0.
Description Mm CTX Male Treated #7
Data processing Data were analyzed using GeneSpring software v. 11.0.2. Raw data were imported into GeneSpring and normalized using global normalization.
 
Submission date Aug 04, 2011
Last update date Jul 28, 2014
Contact name JC xiao
E-mail(s) jxiao4@jhmi.edu
Organization name Johns Hopkins University
Street address 600 N. Wolfe Street, Blalock 1109
City Baltimore
State/province MD
ZIP/Postal code 21287
Country USA
 
Platform ID GPL6885
Series (1)
GSE31207 The Sex-Specific Changes in Gene Expression and Behavior Induced by Chronic Toxoplasma Infection in Mice

Data table header descriptions
ID_REF
VALUE The normalized data were used to identify changes in gene expression in the 2 group comparisons: male infected vs male control and female infected vs female control.

Data table
ID_REF VALUE
ILMN_2607609 0.35941792
ILMN_1238674 0
ILMN_3062534 -0.13777113
ILMN_3140158 0.16682816
ILMN_2674533 -0.107931614
ILMN_2680307 -0.11097622
ILMN_2935307 0
ILMN_2910106 0.19360304
ILMN_1215893 0.050881863
ILMN_2732092 -0.3357358
ILMN_2603725 0.07096577
ILMN_2664884 0.44967747
ILMN_3003575 0
ILMN_1229397 0.17343521
ILMN_1225873 0.16719246
ILMN_2899591 -0.016170979
ILMN_2658355 0
ILMN_2682763 0.42878056
ILMN_2521511 0
ILMN_2691286 0.6780719

Total number of rows: 25697

Table truncated, full table size 547 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap