|
| Status |
Public on Jul 28, 2014 |
| Title |
frontal cortices_male infection_6 month_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
male infection_frontal cortices
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
gender: Male
tissue: Frontal Cortex
infection: 400 tachyzoites of Toxoplasma pru strain
|
| Treatment protocol |
Mice of each gender were either mock-infected with sterile DPBS or infected with 400 tachyzoites (2 parasites/μl) intraperitoneally.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using the RNeasy reagent . The RNA concentration and integrity was determined using Agilent BioAnalyzer.
|
| Label |
biotin
|
| Label protocol |
Total RNA was used to generate biotin labeled cRNA using the Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Biotinylated cRNAs were purified, fragmented, and subsequently hybridized to Illumina Mouse Ref-8 v2.0 Expression BeadChips. Each BeadChip has 24,000 well-annotated RefSeq transcripts with approximately 30-fold redundancy.
|
| Scan protocol |
The arrays were scanned using an Illumina BeadStation 500X Genetic Analysis Systems scanner and the image data extracted using Illumina GenomeStudio software, version 3.0.
|
| Description |
Mm CTX Male Treated #8
|
| Data processing |
Data were analyzed using GeneSpring software v. 11.0.2. Raw data were imported into GeneSpring and normalized using global normalization.
|
| |
|
| Submission date |
Aug 04, 2011 |
| Last update date |
Jul 28, 2014 |
| Contact name |
JC xiao |
| E-mail(s) |
jxiao4@jhmi.edu
|
| Organization name |
Johns Hopkins University
|
| Street address |
600 N. Wolfe Street, Blalock 1109
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31207 |
The Sex-Specific Changes in Gene Expression and Behavior Induced by Chronic Toxoplasma Infection in Mice |
|
