TRIzol reagent (Invitrogen) followed by oligo(dT) cellulose (Ambion)
Description
Cardiac fibroblasts were isolated from 3-month old C57BL/6 mice. RNA was prepared from quiescent, serum-starved cardiac fibroblasts in passage 15. A total number of 118654 tags was sequenced. After removal of duplicate ditags and linker-derived sequences 110169 tags remained. Keywords = cardiac Keywords = fibroblast Keywords = mouse Keywords = heart