|
| Status |
Public on Jun 30, 2007 |
| Title |
Calf 162 (salmonella) ab T cells time 48 |
| Sample type |
RNA |
| |
|
| Source name |
ab T cells
|
| Organism |
Bos taurus |
| Characteristics |
ab T cells derived from lymphatic fluid
|
| Extracted molecule |
total RNA |
| Extraction protocol |
lymphatic cells were stained with GD3.8 directly conjugated to FITC, washed, and sorted on a Vantage SE cell sorter (BD Immunocytometry Systems) as previously described, suspended in Buffer RLT and immediately lysed using Qiashredder columns then frozen at -80°C. RNA was extracted following the manufacturer’s protocol for RNeasy (Qiagen) column purification.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified using Affymetrix One-cycle protocol with approximately 1.6 micrograms of total RNA as described in the GeneChip® Expression Analysis Technical Manual (June 2004).
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
ab T cells derived from lymphatic fluid of calf 162 (salmonella infection) at time 48hr post infection
|
| Data processing |
Affymetrix GeneChip Operating Software V.1.2
|
| |
|
| Submission date |
Oct 12, 2005 |
| Last update date |
Dec 21, 2006 |
| Contact name |
Jodi Fern Hedges |
| E-mail(s) |
jodi.hedges@gmail.com
|
| Phone |
406-994-6384
|
| Organization name |
Montana State University
|
| Department |
Veterinary Molecular Biology
|
| Lab |
Jutila
|
| Street address |
960 Technology Blvd.
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59718 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE3439 |
Profiling of mucosal derived bovine ab and gd T cells during Salmonella enterica serovar typhimurium enterocolitis |
|
