|
Status |
Public on Jun 30, 2007 |
Title |
Calf 162 (salmonella) ab T cells time 48 |
Sample type |
RNA |
|
|
Source name |
ab T cells
|
Organism |
Bos taurus |
Characteristics |
ab T cells derived from lymphatic fluid
|
Extracted molecule |
total RNA |
Extraction protocol |
lymphatic cells were stained with GD3.8 directly conjugated to FITC, washed, and sorted on a Vantage SE cell sorter (BD Immunocytometry Systems) as previously described, suspended in Buffer RLT and immediately lysed using Qiashredder columns then frozen at -80°C. RNA was extracted following the manufacturer’s protocol for RNeasy (Qiagen) column purification.
|
Label |
biotin
|
Label protocol |
RNA was amplified using Affymetrix One-cycle protocol with approximately 1.6 micrograms of total RNA as described in the GeneChip® Expression Analysis Technical Manual (June 2004).
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
ab T cells derived from lymphatic fluid of calf 162 (salmonella infection) at time 48hr post infection
|
Data processing |
Affymetrix GeneChip Operating Software V.1.2
|
|
|
Submission date |
Oct 12, 2005 |
Last update date |
Dec 21, 2006 |
Contact name |
Jodi Fern Hedges |
E-mail(s) |
jodi.hedges@gmail.com
|
Phone |
406-994-6384
|
Organization name |
Montana State University
|
Department |
Veterinary Molecular Biology
|
Lab |
Jutila
|
Street address |
960 Technology Blvd.
|
City |
Bozeman |
State/province |
MT |
ZIP/Postal code |
59718 |
Country |
USA |
|
|
Platform ID |
GPL2112 |
Series (1) |
GSE3439 |
Profiling of mucosal derived bovine ab and gd T cells during Salmonella enterica serovar typhimurium enterocolitis |
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