|
| Status |
Public on Apr 10, 2012 |
| Title |
Stab5b transgenic F1 2N |
| Sample type |
RNA |
| |
|
| Source name |
Stab5b transgenic
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: B6 NOD mixed
age: 4 wk
gender: male
cell type: CD8+ thymocyte
genotype: Stab5b transgenic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy minikit
|
| Label |
Cy3
|
| Label protocol |
Total RNA (200 ng) was converted into biotin labeled cRNA using Target Amp cDNA synthesis kit (Epicenter catalog no. TAB1R6924).
|
| |
|
| Hybridization protocol |
Approximately 750ng of labeled cRNA was fragmented, and hybridized to the Illumina MouseRef-8 v2.0 Expression beadchips in accordance to the Illumina technical manual. RNA from each culture was independently analyzed.
|
| Scan protocol |
After hybridization, arrays were washed, stained with Cy3-conjugated streptavidin, and scanned. Image data were analyzed with BeadStudio V2.0.
|
| Description |
B6.NODF1
|
| Data processing |
The raw data were normalized using Robust Spline Normalization.
|
| |
|
| Submission date |
Aug 18, 2011 |
| Last update date |
Apr 10, 2012 |
| Contact name |
Jin-Xiong She |
| E-mail(s) |
jshe@gru.edu
|
| Phone |
706-721-3410
|
| Fax |
706-721-3688
|
| URL |
http://www.cbgm.mcg.edu/
|
| Organization name |
Medical College of Georgia
|
| Department |
Center for Biotechnology and Genomic Medicine
|
| Street address |
1120 15th Street, CA4124
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912-2400 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31526 |
Exacerbated T cell lymphoma in NOD.Stat5bTg mice is caused by selective activation of Stat5b in CD8+ thymocytes and can be prevented by inhibition of the Stat5b signaling pathway with chemoprevention agents |
|
