|
| Status |
Public on Dec 31, 2014 |
| Title |
control_CD34Neg_3 |
| Sample type |
RNA |
| |
|
| Source name |
CD34Neg
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
cell type: CD34Neg
genotype/variation: control (IFNgamma-/-)
|
| Treatment protocol |
For analysis of hematopoietic progenitors by flow cytometry, bone marrow cells were stained with antibodies against lineage markers, CD34, Flt3, Sca-1, c-Kit, Cd16/32 (93), IL-7Rα (A7R34), CD48 (HM-48-1), CD150 (TCF15-12F12.2, Biolegend) and CD27 (LG.7F9) and identified as: LT-HSC (Lin-c-Kit+Sca-1+CD34-Flt3-), ST-HSC (Lin-c-Kit+Sca-1+CD34-Flt3-)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Plus micro kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified with Ambion MessageAmp kit for Illumina arrays and biotinylated during a second round of amplification with the same kit
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
FACS pools of CD34 negative cells obtained from two mice
|
| Data processing |
Beadarray v1.3 probe summarization; vsn normalization
Matrix non-normalized: Two columns per sample. First column contains intensity values; second column contains detection p-value, is low when intensity value is above background threshold. Matrix normalized: Two columns per sample. First column contains vsn normalized intensity values; second column contains same detection p-values as non-normalized data
|
| |
|
| Submission date |
Aug 22, 2011 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Peter A.C. 't Hoen |
| E-mail(s) |
p.a.c.hoen@lumc.nl
|
| Phone |
+31 71 5269421
|
| Fax |
+31 71 5268285
|
| URL |
http://www.humgen.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Center for Human and Clinical Genetics
|
| Street address |
PO Box 9600
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31574 |
Genome-wide expression analysis of self renewing hematopoietic stem cells (HSC) in control and CD70TG mice. |
|
