NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM785598 Query DataSets for GSM785598
Status Public on Sep 01, 2011
Title H1975F
Sample type RNA
 
Source name NSCLC cell line
Organism Homo sapiens
Characteristics cell type: non small cell lung cancer cell (NSCLC)
erlotinib sensitivity: No
Treatment protocol none
Growth protocol NSCLC cell lines were cultured for 3d in 10% serum-containing RPMI 1640 with no drug treatment.
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen Rneasy column preparation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 24, 2011
Last update date Sep 01, 2016
Contact name Esther P Black
E-mail(s) penni.black@uky.edu
Phone 859-323-5898
Fax 859-257-7564
Organization name University of Kentucky
Department Pharmaceutical Sciences
Street address 789 S Limestone
City Lexington
State/province KY
ZIP/Postal code 40536
Country USA
 
Platform ID GPL96
Series (1)
GSE31625 Gene Expression Patterns that Predict Sensitivity to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Lung Cancer Cell Lines and Human Lung Tumors
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE MAS5 Signal

Data table
ID_REF VALUE
200000_s_at 2939.1
200001_at 2411.8
200002_at 16343.6
200003_s_at 30678.5
200004_at 7718.1
200005_at 5372.4
200006_at 8425.4
200007_at 6362.3
200008_s_at 3799.5
200009_at 6785.6
200010_at 13957.2
200011_s_at 916.3
200012_x_at 10591.5
200013_at 16639.7
200014_s_at 4211.7
200015_s_at 2539.5
200016_x_at 12628.7
200017_at 14977.1
200018_at 20879.8
200019_s_at 13899.4

Total number of rows: 22215

Table truncated, full table size 354 Kbytes.




Supplementary file Size Download File type/resource
GSM785598_H1975F.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap