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Sample GSM785608 Query DataSets for GSM785608
Status Public on Sep 01, 2011
Title PC-9_8
Sample type RNA
 
Source name NSCLC cell line
Organism Homo sapiens
Characteristics cell type: non small cell lung cancer cell (NSCLC)
erlotinib sensitivity: Yes
Treatment protocol none
Growth protocol NSCLC cell lines were cultured for 3d in 10% serum-containing RPMI 1640 with no drug treatment.
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen Rneasy column preparation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 24, 2011
Last update date Sep 01, 2016
Contact name Esther P Black
E-mail(s) penni.black@uky.edu
Phone 859-323-5898
Fax 859-257-7564
Organization name University of Kentucky
Department Pharmaceutical Sciences
Street address 789 S Limestone
City Lexington
State/province KY
ZIP/Postal code 40536
Country USA
 
Platform ID GPL96
Series (1)
GSE31625 Gene Expression Patterns that Predict Sensitivity to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Lung Cancer Cell Lines and Human Lung Tumors
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE MAS5 Signal

Data table
ID_REF VALUE
200000_s_at 3094.7
200001_at 4389.4
200002_at 13280.7
200003_s_at 21452.7
200004_at 6464.7
200005_at 7811.8
200006_at 6557.5
200007_at 6753.8
200008_s_at 3260.2
200009_at 7005.3
200010_at 9814.5
200011_s_at 2989.3
200012_x_at 15496.3
200013_at 20094.4
200014_s_at 2840.4
200015_s_at 5216.5
200016_x_at 10614.2
200017_at 20989.1
200018_at 17490.2
200019_s_at 16594.2

Total number of rows: 22215

Table truncated, full table size 353 Kbytes.




Supplementary file Size Download File type/resource
GSM785608_PC-9_8.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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