|
| Status |
Public on Aug 25, 2011 |
| Title |
SHED TP53-transfected 2 |
| Sample type |
RNA |
| |
|
| Source name |
SHED TP53-transfected
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: stem cells from human exfoliated decidiuous teeth
|
| Treatment protocol |
Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmids for SP1, TP53 and as control an empty vector without an insert.
|
| Growth protocol |
Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with biotin-labeled cRNA in a hybridization oven 640
|
| Scan protocol |
GeneTitan scanner
|
| Description |
dental progenitor/stem cells
Gene expression of SHED after 48 hours of transfection with a TP53 expression vector
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software.
For the creation of the summarized log-signal the robust multi-chip analysis (RAM) algorithm was used.
|
| |
|
| Submission date |
Aug 24, 2011 |
| Last update date |
Sep 23, 2011 |
| Contact name |
Christian Morsczeck |
| E-mail(s) |
christian.morsczeck@klinik.uni-regensburg.de
|
| Organization name |
University of Regensburg
|
| Street address |
Franz-Josef Strauss Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE31628 |
Gene expression profiles of DFCs and SHED 48 hours after in vitro transfection with a TP53 plasmid, a SP1 plasmid, or an empty vector. |
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