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Sample GSM786619 Query DataSets for GSM786619
Status Public on Apr 23, 2012
Title WT eWAT HFD-B
Sample type RNA
 
Source name wild-type mouse eWAT on HFD
Organism Mus musculus
Characteristics genotype/variation: wild-type
age: 24 weeks old
treatment: High-fat diet
tissue: epidydimal white adipose tissue
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description wild-type mouse eWAT on HFD, technical replicate 2
Data processing The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
 
Submission date Aug 26, 2011
Last update date Apr 23, 2012
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL6885
Series (1)
GSE31692 A PPARĪ³-FGF1 axis is required for adaptive adipose remodeling

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 4625.537 0
ILMN_2721178 1081.082 0
ILMN_3033922 2262.048 0
ILMN_3092673 12180.03 0
ILMN_2816356 122.4478 0.08897243
ILMN_2808939 649.6116 0
ILMN_2634564 366.1065 0
ILMN_2737647 116.4745 0.1403509
ILMN_2734484 581.2686 0
ILMN_2952292 207.7137 0
ILMN_2699078 101.9606 0.6403509
ILMN_1213681 491.8294 0
ILMN_2735413 119.1572 0.1152882
ILMN_2735415 193.563 0
ILMN_2891688 768.1406 0
ILMN_2637698 2302.511 0
ILMN_2674228 366.9836 0
ILMN_2601546 106.0788 0.4398496
ILMN_1230831 99.76018 0.7468672
ILMN_2848071 127.7657 0.05137845

Total number of rows: 25697

Table truncated, full table size 716 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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