|
| Status |
Public on Jan 01, 2012 |
| Title |
IL-10-/- with Pathology = 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
IL-10-/- Colon
|
| Organism |
Mus musculus |
| Characteristics |
strain background: BALB/c
genotype/variation: IL-10 -/-
tissue: colon
pathology: mild pathology
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled using the miRCURY Hy3/Hy5 Power labeling kit following the manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
Common Reference (Samples 1, 2, and 3)
|
| Organism |
Mus musculus |
| Characteristics |
strain background: BALB/c
tissue: colon
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy following the manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Samples were labeled using the miRCURY Hy3/Hy5 Power labeling kit following the manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized using a Tecan HS4800™ Pro automated hybridization station
|
| Scan protocol |
Agilent G2505B Microarray Scanners
|
| Description |
Mild Pathology. Pooled total RNA from 3 mice.
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Green signal/processed Red signal. Agilent software was used.
|
| |
|
| Submission date |
Aug 29, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Jeremy S Schaefer |
| E-mail(s) |
Jeremy.Schaefer@uth.tmc.edu
|
| Phone |
713-486-4107
|
| Organization name |
The University of Texas Health Science Center at Houston School of Dentistry
|
| Department |
Diagnostic Sciences
|
| Lab |
John Klein
|
| Street address |
1941 East Rd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE31706 |
Murine colon: IL-10-/- versus BALB/c |
|
