|
Status |
Public on Jan 01, 2012 |
Title |
IL-10-/- with Pathology > 3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
IL-10-/- Colon
|
Organism |
Mus musculus |
Characteristics |
strain background: BALB/c genotype/variation: IL-10 -/- tissue: colon pathology: severe pathology
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy following the manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Samples were labeled using the miRCURY Hy3/Hy5 Power labeling kit following the manufacturer's instructions
|
|
|
Channel 2 |
Source name |
Common Reference (Samples 1, 2, and 3)
|
Organism |
Mus musculus |
Characteristics |
strain background: BALB/c tissue: colon
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy following the manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
Samples were labeled using the miRCURY Hy3/Hy5 Power labeling kit following the manufacturer's instructions
|
|
|
|
Hybridization protocol |
Samples were hybridized using a Tecan HS4800™ Pro automated hybridization station
|
Scan protocol |
Agilent G2505B Microarray Scanners
|
Description |
Severe Pathology. Pooled total RNA from 3 mice.
|
Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Green signal/processed Red signal. Agilent software was used.
|
|
|
Submission date |
Aug 29, 2011 |
Last update date |
Jan 01, 2012 |
Contact name |
Jeremy S Schaefer |
E-mail(s) |
Jeremy.Schaefer@uth.tmc.edu
|
Phone |
713-486-4107
|
Organization name |
The University of Texas Health Science Center at Houston School of Dentistry
|
Department |
Diagnostic Sciences
|
Lab |
John Klein
|
Street address |
1941 East Rd
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77054 |
Country |
USA |
|
|
Platform ID |
GPL7723 |
Series (1) |
GSE31706 |
Murine colon: IL-10-/- versus BALB/c |
|