|
| Status |
Public on Apr 23, 2013 |
| Title |
WT CD4+ T cells aCD3 24h technical Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
total CD4+
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: CD4+ T cell
treatment: aCD3
time: 24h
|
| Treatment protocol |
CD4+ T cells were activated with plate-bound (0.1μg/ml) αCD3 (145-2C11) for 4 hours or 24 hours in 96 well U bottom plates.
|
| Growth protocol |
Cells were incubated in complete RPMI medium at 37 degree C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Qiagen Total RNA isolation kit.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Array Station (GCAS) HT system
|
| Scan protocol |
standard Affymetrix HT Scanner protocol
|
| Description |
Gene expression data of splenic total CD4+ T cells after 24h aCD3 stimulation
|
| Data processing |
Array quality was assessed using the R/Bioconductor package. All arrays passed visual inspection and no technical outliers were identified. Raw CEL files were processed using the robust multiarray average (RMA) algorithm.
|
| |
|
| Submission date |
Aug 29, 2011 |
| Last update date |
Apr 23, 2013 |
| Contact name |
Marton Keszei |
| E-mail(s) |
marton.keszei@ki.se
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Medicine
|
| Lab |
Immunology/Cox Terhorst
|
| Street address |
3 Blackfan Cir., CLS building room 928
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE31733 |
Expression data from B6, Sle1b and Sle1b.BACSlamf6-H1 CD4+ T cells |
|
