|
| Status |
Public on Sep 03, 2011 |
| Title |
A CD34+ VSEL |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ VSEL
|
| Organism |
Homo sapiens |
| Characteristics |
isolation source: umbilical cord blood
cell type: very small embryonic-like (VSEL) cells
surface markers: Lin- CD45- CXCR4+ CD34+
|
| Treatment protocol |
hVSEL were FACS sorted (BD Vantage SE) from lineage-depleted (MACS) total nucleated human umbilical cord blood cells after NH4Cl lysis. Two populations of hVSEL cells were sorted: Lin-CD45-CXCR4+CD34- and Lin-CD45-CXCR4+CD34+. Dead cells and non-nucleated cells were excluded through Hoechst 33342 and propidium iodid staining.
|
| Growth protocol |
primary FACS sorted cells; not cultured
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Rneasy kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
RNA labelling was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using NuGen Pico labelling kit (NuGen, San Carlos, USA)
|
| |
|
| Hybridization protocol |
RNA hybridisation and microarray analysis was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Affymetrix GeneTitan Human Genome U219 microarray (Affymetrix, Santa Clara, USA)
|
| Scan protocol |
RNA hybridisation and microarray analysis was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Affymetrix GeneTitan Human Genome U219 microarray (Affymetrix, Santa Clara, USA)
|
| Description |
A982-02.plier-pm-only-Signal
|
| Data processing |
The data was preprocessed and analysed using R/Bioconductor and Tigr MeV Suite.
|
| |
|
| Submission date |
Sep 02, 2011 |
| Last update date |
Sep 03, 2011 |
| Contact name |
Andreas Heider |
| E-mail(s) |
aheider@trm.uni-leipzig.de
|
| Organization name |
Universität Leipzig
|
| Department |
Biologie
|
| Lab |
TRM Leipzig
|
| Street address |
Semmelweisstr. 14
|
| City |
Leipzig |
| State/province |
Bayern |
| ZIP/Postal code |
04103 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE31869 |
Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics |
|
