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Sample GSM790418 Query DataSets for GSM790418
Status Public on Sep 03, 2011
Title B CD34+ VSEL
Sample type RNA
 
Source name CD34+ VSEL
Organism Homo sapiens
Characteristics isolation source: umbilical cord blood
cell type: very small embryonic-like (VSEL) cells
surface markers: Lin- CD45- CXCR4+ CD34+
Treatment protocol hVSEL were FACS sorted (BD Vantage SE) from lineage-depleted (MACS) total nucleated human umbilical cord blood cells after NH4Cl lysis. Two populations of hVSEL cells were sorted: Lin-CD45-CXCR4+CD34- and Lin-CD45-CXCR4+CD34+. Dead cells and non-nucleated cells were excluded through Hoechst 33342 and propidium iodid staining.
Growth protocol primary FACS sorted cells; not cultured
Extracted molecule total RNA
Extraction protocol RNA extraction was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Rneasy kit (Qiagen, Hilden, Germany)
Label biotin
Label protocol RNA labelling was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using NuGen Pico labelling kit (NuGen, San Carlos, USA)
 
Hybridization protocol RNA hybridisation and microarray analysis was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Affymetrix GeneTitan Human Genome U219 microarray (Affymetrix, Santa Clara, USA)
Scan protocol RNA hybridisation and microarray analysis was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Affymetrix GeneTitan Human Genome U219 microarray (Affymetrix, Santa Clara, USA)
Description A982-04.plier-pm-only-Signal
Data processing The data was preprocessed and analysed using R/Bioconductor and Tigr MeV Suite.
 
Submission date Sep 02, 2011
Last update date Sep 03, 2011
Contact name Andreas Heider
E-mail(s) aheider@trm.uni-leipzig.de
Organization name Universität Leipzig
Department Biologie
Lab TRM Leipzig
Street address Semmelweisstr. 14
City Leipzig
State/province Bayern
ZIP/Postal code 04103
Country Germany
 
Platform ID GPL13667
Series (1)
GSE31869 Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics

Data table header descriptions
ID_REF
VALUE PLIER summarized (PM-only), quantile normalized data are provided

Data table
ID_REF VALUE
11715100_at 44.81256
11715101_s_at 39.23711
11715102_x_at 40.49586
11715103_x_at 114.9851
11715104_s_at 39.73949
11715105_at 30.40972
11715106_x_at 31.94741
11715107_s_at 41.92636
11715108_x_at 34.99145
11715109_at 32.26093
11715110_at 38.88155
11715111_s_at 44.02054
11715112_at 31.68874
11715113_x_at 44.25148
11715114_x_at 35.64809
11715115_s_at 27.55692
11715116_s_at 427.5266
11715117_x_at 43.6501
11715118_s_at 288.9907
11715119_s_at 60.87538

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM790418_A982-04.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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