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Sample GSM794115 Query DataSets for GSM794115
Status Public on Feb 16, 2012
Title Sort-purified (CD8/CD44low) naïve transgenic CD8 T cells replicate#3
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAG transgenic CD8
cd8 t cell subsets: Naïve
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description Naïve T cells were isolated from spleens of transgenic (naïve) TCR GAG mice and FACS sorted
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
 
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
 
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
ID_REF
VALUE log2 transformed, quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 5.856321771
ILMN_1212612 5.761285273
ILMN_1212619 5.668081294
ILMN_1212628 5.441394946
ILMN_1212632 5.984741237
ILMN_1212636 10.045473
ILMN_1212637 7.295416921
ILMN_1212645 5.776103988
ILMN_1212648 8.900746075
ILMN_1212653 7.072748952
ILMN_1212672 8.497359547
ILMN_1212682 6.053618219
ILMN_1212683 5.517695631
ILMN_1212685 5.758267639
ILMN_1212692 5.73804721
ILMN_1212693 7.577856789
ILMN_1212695 5.817282155
ILMN_1212698 5.704871964
ILMN_1212702 7.301099838
ILMN_1212703 6.720916846

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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