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Sample GSM794117 Query DataSets for GSM794117
Status Public on Feb 16, 2012
Title Sort-purified (CD44hi/CD62Lhi) memory transgenic CD8 T cells replicate #2
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAG transgenic CD8
cd8 t cell subsets: Memory
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description Memory T cells were generated by transfer of 1x10^6 naïve, transgenic TCR GAG CD8 T cells into B6 mice, followed by i.p. infection one day later with 3x10^7 cfu GAG-expressing Listeria monocytogenes . Splenic CD44hi, CD62Lhi memory TCRGAG T cells were sorted at least two months post infection.
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
 
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
 
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
ID_REF
VALUE log2 transformed, quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 5.852331831
ILMN_1212612 5.612352499
ILMN_1212619 5.496547567
ILMN_1212628 5.578737486
ILMN_1212632 5.755421735
ILMN_1212636 9.680933791
ILMN_1212637 7.273329387
ILMN_1212645 5.738587714
ILMN_1212648 9.34069578
ILMN_1212653 7.031953862
ILMN_1212672 8.916675678
ILMN_1212682 5.900786321
ILMN_1212683 5.59275601
ILMN_1212685 5.842978832
ILMN_1212692 5.792508342
ILMN_1212693 7.288758435
ILMN_1212695 5.686313758
ILMN_1212698 5.589364014
ILMN_1212702 9.096767855
ILMN_1212703 6.853829352

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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