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Sample GSM794118 Query DataSets for GSM794118
Status Public on Feb 16, 2012
Title Sort-purified (CD44hi/CD62Lhi) memory transgenic CD8 T cells replicate #3
Sample type RNA
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAG transgenic CD8
cd8 t cell subsets: Memory
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description Memory T cells were generated by transfer of 1x10^6 naïve, transgenic TCR GAG CD8 T cells into B6 mice, followed by i.p. infection one day later with 3x10^7 cfu GAG-expressing Listeria monocytogenes . Splenic CD44hi, CD62Lhi memory TCRGAG T cells were sorted at least two months post infection.
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
VALUE log2 transformed, quantile normalized

Data table
ILMN_1212607 5.827480324
ILMN_1212612 5.513806537
ILMN_1212619 5.618336555
ILMN_1212628 5.726467955
ILMN_1212632 5.552745937
ILMN_1212636 10.10447645
ILMN_1212637 7.691906329
ILMN_1212645 5.75595577
ILMN_1212648 9.277411398
ILMN_1212653 7.485104761
ILMN_1212672 8.43359935
ILMN_1212682 6.068814099
ILMN_1212683 5.714245518
ILMN_1212685 5.762083004
ILMN_1212692 5.843816391
ILMN_1212693 7.725082179
ILMN_1212695 5.723012396
ILMN_1212698 5.71057687
ILMN_1212702 10.3100068
ILMN_1212703 6.757378897

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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