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Sample GSM794119 Query DataSets for GSM794119
Status Public on Feb 16, 2012
Title Sort-purified (CD44hi) tolerant transgenic CD8 T cells replicate #1
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAGxAlb:GAG transgenic CD8
cd8 t cell subsets: Tolerant
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description Tolerant T cells were isolated from spleens of transgenic tolerant TCR GAG xAlb:GAG mice and FACS sorted
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
 
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
 
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
ID_REF
VALUE log2 transformed, quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 5.735522177
ILMN_1212612 5.695993813
ILMN_1212619 5.689485543
ILMN_1212628 5.615298579
ILMN_1212632 5.735522177
ILMN_1212636 10.08662279
ILMN_1212637 7.305423389
ILMN_1212645 5.99034983
ILMN_1212648 8.676497795
ILMN_1212653 6.737326223
ILMN_1212672 8.838195439
ILMN_1212682 5.994881385
ILMN_1212683 5.683134985
ILMN_1212685 5.752391798
ILMN_1212692 5.735522177
ILMN_1212693 7.346484185
ILMN_1212695 5.792508342
ILMN_1212698 5.631104282
ILMN_1212702 12.18401368
ILMN_1212703 6.877826033

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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