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Sample GSM794121 Query DataSets for GSM794121
Status Public on Feb 16, 2012
Title Sort-purified (CD44hi) tolerant transgenic CD8 T cells replicate #3
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAGxAlb:GAG transgenic CD8
cd8 t cell subsets: Tolerant
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description Tolerant T cells were isolated from spleens of transgenic tolerant TCR GAG xAlb:GAG mice and FACS sorted
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
 
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
 
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
ID_REF
VALUE log2 transformed, quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 5.741466986
ILMN_1212612 5.671293372
ILMN_1212619 5.590162849
ILMN_1212628 5.488000771
ILMN_1212632 5.845490051
ILMN_1212636 10.40127776
ILMN_1212637 8.018700931
ILMN_1212645 5.938599455
ILMN_1212648 8.78260514
ILMN_1212653 6.762525996
ILMN_1212672 8.71975387
ILMN_1212682 6.011972642
ILMN_1212683 5.798396195
ILMN_1212685 5.652868868
ILMN_1212692 5.730458815
ILMN_1212693 7.685776664
ILMN_1212695 5.763765654
ILMN_1212698 5.606244802
ILMN_1212702 11.19614308
ILMN_1212703 6.767919586

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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