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Sample GSM794122 Query DataSets for GSM794122
Status Public on Feb 16, 2012
Title Sort-purified rescued transgenic CD8 T cells from lymphopenic tolerogenic mouse replicate #1
Sample type RNA
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAGxAlb:GAG transgenic CD8
cd8 t cell subsets: Tolerant T cells undergoing homeostatic proliferation in lymphopenic, tolerogenic mouse, 20-22 days post adoptive transfer
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description rescued T cells are defined in this study as tolerant T cells undergoing homeostaic proliferation in lymphopenic, tolerogenic Alb:GAG mice.
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
VALUE log2 transformed, quantile normalized

Data table
ILMN_1212607 5.896917639
ILMN_1212612 5.725741157
ILMN_1212619 5.767301673
ILMN_1212628 5.552745937
ILMN_1212632 5.798396195
ILMN_1212636 10.24613886
ILMN_1212637 7.745685527
ILMN_1212645 6.110752988
ILMN_1212648 9.004744817
ILMN_1212653 6.652056204
ILMN_1212672 8.270326477
ILMN_1212682 6.018663844
ILMN_1212683 5.536674618
ILMN_1212685 5.773117223
ILMN_1212692 6.288358562
ILMN_1212693 7.592556702
ILMN_1212695 6.145133965
ILMN_1212698 5.695622702
ILMN_1212702 14.67810346
ILMN_1212703 6.645442282

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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