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Sample GSM794123 Query DataSets for GSM794123
Status Public on Feb 16, 2012
Title Sort-purified rescued transgenic CD8 T cells from lymphopenic tolerogenic mouse replicate #2
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAGxAlb:GAG transgenic CD8
cd8 t cell subsets: Tolerant T cells undergoing homeostatic proliferation in lymphopenic, tolerogenic mouse, 20-22 days post adoptive transfer
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description rescued T cells are defined in this study as tolerant T cells undergoing homeostaic proliferation in lymphopenic, tolerogenic Alb:GAG mice.
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
 
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
 
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
ID_REF
VALUE log2 transformed, quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 5.852664748
ILMN_1212612 5.593254161
ILMN_1212619 5.761639875
ILMN_1212628 5.660400052
ILMN_1212632 5.587565018
ILMN_1212636 10.27509243
ILMN_1212637 7.523091435
ILMN_1212645 6.004501392
ILMN_1212648 9.100399529
ILMN_1212653 6.95388602
ILMN_1212672 8.280415078
ILMN_1212682 6.208283138
ILMN_1212683 5.798914559
ILMN_1212685 5.726104602
ILMN_1212692 6.304754391
ILMN_1212693 7.452858965
ILMN_1212695 6.125843933
ILMN_1212698 5.838783728
ILMN_1212702 12.89072407
ILMN_1212703 6.7322692

Total number of rows: 25697

Table truncated, full table size 625 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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