cd8 t cells: TCR GAGxAlb:GAG transgenic CD8 cd8 t cell subsets: Tolerant T cells undergoing homeostatic proliferation in lymphopenic, tolerogenic mouse, 20-22 days post adoptive transfer
Extracted molecule
total RNA
Extraction protocol
After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
