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Sample GSM794124 Query DataSets for GSM794124
Status Public on Feb 16, 2012
Title Sort-purified rescued transgenic CD8 T cells from lymphopenic tolerogenic mouse replicate #3
Sample type RNA
Source name mouse splenocytes
Organism Mus musculus
Characteristics cd8 t cells: TCR GAGxAlb:GAG transgenic CD8
cd8 t cell subsets: Tolerant T cells undergoing homeostatic proliferation in lymphopenic, tolerogenic mouse, 20-22 days post adoptive transfer
Extracted molecule total RNA
Extraction protocol After FACS-sorting , T-cells were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems)
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned on an Illumina BeadArray Reader
Description rescued T cells are defined in this study as tolerant T cells undergoing homeostaic proliferation in lymphopenic, tolerogenic Alb:GAG mice.
Data processing The data was quantile normalized using the Bioconductor package, “lumi,” and transformed to log2 scale
Submission date Sep 09, 2011
Last update date Feb 16, 2012
Contact name Andrea Schietinger
Organization name University of Washington
Department Immunology
Lab Dr. Philip D Greenberg
Street address 1959 NE Pacific Street
City Seattle
State/province WA
ZIP/Postal code 98195-7650
Country USA
Platform ID GPL6885
Series (1)
GSE32025 Gene expression profile of murine tolerant, self-antigen specific CD8 T cells

Data table header descriptions
VALUE log2 transformed, quantile normalized

Data table
ILMN_1212607 5.854993017
ILMN_1212612 5.671293372
ILMN_1212619 5.690603334
ILMN_1212628 5.771005189
ILMN_1212632 5.75595577
ILMN_1212636 10.30475439
ILMN_1212637 7.580421894
ILMN_1212645 5.990274183
ILMN_1212648 9.239876938
ILMN_1212653 6.780266349
ILMN_1212672 8.364630793
ILMN_1212682 6.225801909
ILMN_1212683 5.725195817
ILMN_1212685 5.765004246
ILMN_1212692 5.841134489
ILMN_1212693 7.401903472
ILMN_1212695 5.676944359
ILMN_1212698 5.504832226
ILMN_1212702 10.59797183
ILMN_1212703 6.465974465

Total number of rows: 25697

Table truncated, full table size 625 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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