|
| Status |
Public on Oct 03, 2012 |
| Title |
CTRL 1 week Ly6Chi monocyte #3 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/6J
monocyte subtype: Ly6Chi
treatment: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted samples were pelleted and immediately resuspended in TRIzol (Invitrogen, Carlsbad, CA) and stored at -80˚C until RNA isolation. RNA was isolated with RNeasy Micro-RNA isolation kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
RNA amplification, direct labeling, hybridization, and slide scanning were carried out as described in Grabner R, 2009, with slight modifications. We enhanced the in vitro transcription-time from 4 h to 64 h and added fresh nucleotides and T7 polymerase alternating every 8 h and 16 h.
|
| |
|
| Hybridization protocol |
as described in Grabner R, 2009
|
| Scan protocol |
as described in Grabner R, 2009
|
| Description |
Ly6Chi #3
|
| Data processing |
All arrays were analysed with ArrayAssist (Stratagene). We used GC-RMA, and then compared the two subsets within each treatment using an unpaired T test with asymptotic P value computation and Benjamini-Hochburg correction.
|
| |
|
| Submission date |
Sep 09, 2011 |
| Last update date |
Oct 03, 2012 |
| Contact name |
Emmanuel GAUTIER |
| E-mail(s) |
emmanuel.gautier@mssm.edu
|
| Organization name |
Mount Sinai School of Medecine
|
| Street address |
1425 Madison ave
|
| City |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32034 |
Tissue-specific differences in PPARγ control of macrophage function. |
|
