|
Status |
Public on Oct 03, 2012 |
Title |
ROSI 1 week Ly6Chi monocyte #2 |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Mus musculus |
Characteristics |
gender: male strain: C57BL/6J monocyte subtype: Ly6Chi treatment: Rosiglitazone (Rosi)
|
Extracted molecule |
total RNA |
Extraction protocol |
Sorted samples were pelleted and immediately resuspended in TRIzol (Invitrogen, Carlsbad, CA) and stored at -80˚C until RNA isolation. RNA was isolated with RNeasy Micro-RNA isolation kit (Qiagen, Valencia, CA).
|
Label |
biotin
|
Label protocol |
RNA amplification, direct labeling, hybridization, and slide scanning were carried out as described in Grabner R, 2009, with slight modifications. We enhanced the in vitro transcription-time from 4 h to 64 h and added fresh nucleotides and T7 polymerase alternating every 8 h and 16 h.
|
|
|
Hybridization protocol |
as described in Grabner R, 2009
|
Scan protocol |
as described in Grabner R, 2009
|
Description |
Rosi Ly6Chi #2
|
Data processing |
All arrays were analysed with ArrayAssist (Stratagene). We used GC-RMA, and then compared the two subsets within each treatment using an unpaired T test with asymptotic P value computation and Benjamini-Hochburg correction.
|
|
|
Submission date |
Sep 09, 2011 |
Last update date |
Oct 03, 2012 |
Contact name |
Emmanuel GAUTIER |
E-mail(s) |
emmanuel.gautier@mssm.edu
|
Organization name |
Mount Sinai School of Medecine
|
Street address |
1425 Madison ave
|
City |
New York |
ZIP/Postal code |
10029 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE32034 |
Tissue-specific differences in PPARγ control of macrophage function. |
|