NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM794248 Query DataSets for GSM794248
Status Public on Oct 03, 2012
Title ROSI 1 week Ly6Chi monocyte #3
Sample type RNA
 
Source name blood
Organism Mus musculus
Characteristics gender: male
strain: C57BL/6J
monocyte subtype: Ly6Chi
treatment: Rosiglitazone (Rosi)
Extracted molecule total RNA
Extraction protocol Sorted samples were pelleted and immediately resuspended in TRIzol (Invitrogen, Carlsbad, CA) and stored at -80˚C until RNA isolation. RNA was isolated with RNeasy Micro-RNA isolation kit (Qiagen, Valencia, CA).
Label biotin
Label protocol RNA amplification, direct labeling, hybridization, and slide scanning were carried out as described in Grabner R, 2009, with slight modifications. We enhanced the in vitro transcription-time from 4 h to 64 h and added fresh nucleotides and T7 polymerase alternating every 8 h and 16 h.
 
Hybridization protocol as described in Grabner R, 2009
Scan protocol as described in Grabner R, 2009
Description Rosi Ly6Chi #3
Data processing All arrays were analysed with ArrayAssist (Stratagene). We used GC-RMA, and then compared the two subsets within each treatment using an unpaired T test with asymptotic P value computation and Benjamini-Hochburg correction.
 
Submission date Sep 09, 2011
Last update date Oct 03, 2012
Contact name Emmanuel GAUTIER
E-mail(s) emmanuel.gautier@mssm.edu
Organization name Mount Sinai School of Medecine
Street address 1425 Madison ave
City New York
ZIP/Postal code 10029
Country USA
 
Platform ID GPL1261
Series (1)
GSE32034 Tissue-specific differences in PPARγ control of macrophage function.

Data table header descriptions
ID_REF
VALUE log2 GC-RMA signal

Data table
ID_REF VALUE
1415670_at 8.09288
1415671_at 10.9485
1415672_at 11.95141
1415673_at 7.723506
1415674_a_at 9.734056
1415675_at 9.467654
1415676_a_at 10.86424
1415677_at 9.823114
1415678_at 10.71973
1415679_at 11.9989
1415680_at 9.391462
1415681_at 10.05858
1415682_at 7.766202
1415683_at 11.83101
1415684_at 8.407242
1415685_at 7.880501
1415686_at 10.51678
1415687_a_at 13.96874
1415688_at 10.55611
1415689_s_at 9.211799

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.




Supplementary file Size Download File type/resource
GSM794248.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap